Springer2012新书《Unnatural Amino Acids: Methods and Protocols 》
Contents
Preface v
Contributors xi
PART I SYNTHESIS OF UNNATURAL AMINO ACIDS
1 Preparation of Unnatural Amino Acids with Ammonia-Lyases and 2,3-Aminomutases 3
2 Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids 21
3 Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates 37
4 Preparation of Glutamate ogues by Enzymatic Transamination 55
5 Carbon–Carbon Bond-Forming Enzymes for the Synthesis of Non-natural Amino Acids 73
6 Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis 87
PART II APPLICATION OF UNNATURAL AMINO ACIDS
7 NMR ysis of Unnatural Amino Acids in Natural Antibiotics 107
8 Site-Specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes 125
9 Application of Unnatural Amino Acids to the De Novo Design of Selective Antibiotic Peptides 135
10 Use of Unnatural Amino Acids to Probe Structure–Activity Relationships and Mode-of-Action of Antimicrobial Peptides 169
Alessandro Tossi, Marco Scocchi, Sotir Zahariev, and Renato Gennaro
PART III USE OF UNNATURAL AMINO ACIDS IN PROTEIN SYNTHESIS
11 Experimental Methods for Scanning Unnatural Amino Acid Mutagenesis 187
12 Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast 199
13 Site-Specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells 215
14 Incorporation of Unnatural Non--Amino Acids into the N-Terminus of Proteins in a Cell-Free Translation System 229
15 Site-Specific Modification of Proteins by the Staudinger-Phosphite Reaction 241
PART IV D-AMINO ACIDS: YSIS AND APPLICATIONS
16 HPLC Methods for Determination of D-Aspartate and N-methyl-D-Aspartate 253
17 Estimation of Chronological Age from the Racemization Rate of L- and D-Aspartic Acid: How to Completely Separate Enantiomers from Dentin 265
18 Enzymatic Detection of D-Amino Acids 273
19 An Enzymatic-HPLC Assay to Monitor Endogenous D-Serine Release from Neuronal Cultures 291
20 Electrophysiological ysis of the Modulation of NMDA-Receptors Function by D-Serine and Glycine in the Central Nervous System 299
21 Biosensors for D-Amino Acid Detection 313
22 ysis of D--Aspartyl Isomers at Specific Sites in Proteins 325
23 Nutritional Value of D-Amino Acids, D-Peptides, and Amino Acid Derivatives in Mice 337
PART V ENZYMES ACTIVE ON D-AMINO ACIDS
24 Preparation and Assay of Recombinant Serine Racemase 357
25 Assay of Amino Acid Racemases 367
26 Assays of D-Amino Acid Oxidases 381
27 Enzymes Acting on D-Amino Acid Containing Peptides 397
Index 407
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